Journal: European journal of medical research

Article Title: Functional analysis and validation of oncodrive gene AP3S1 in ovarian cancer through filtering of mutation data from whole-exome sequencing.

doi: 10.1186/s40001-024-01814-7

Figure Lengend Snippet: Fig. 5 AP3S1 exhibition in ovarian cancer single-cell sequencing results. A Single-cell sequencing results classifying cell subgroups in ovarian cancer tissues. B Cell subgroups identified as immune cell clusters. C Expression level of AP3S1 in immune cell clusters. D Proportions of various immune cells. E Proportions of different immune cells in various ovarian cancer samples. F Expression of AP3S1 in different immune cells. G Expression of DNA repair pathways in cell subgroups. H Expression of oxidative phosphorylation pathways in cell subgroups. I Expression of TGF-β pathways in cell subgroups

Article Snippet: Ovarian cancer cells were subjected to protein extraction for Western blotting analysis after transfection with 80 nM AP3S1-siRNA for 48 h. An activator of the TGF-β signaling pathway known as, SRI-011381 (HY-100347, MCE), was applied at a concentration of 10 μM, and continued treatment was continued for 12 h post-transfection.

Techniques: Single Cell, Sequencing, Expressing, Phospho-proteomics

Journal: European journal of medical research

Article Title: Functional analysis and validation of oncodrive gene AP3S1 in ovarian cancer through filtering of mutation data from whole-exome sequencing.

doi: 10.1186/s40001-024-01814-7

Figure Lengend Snippet: Fig. 10 AP3S1 regulates EMT in ovarian cancer through the TGF-β pathway. A Significant inhibition of TGF-β and EMT pathways in ovarian cancer cells after AP3S1 knockdown. B The TGF-β pathway activator SRI-011381 rescues the decreased expression of TGF-β pathway and EMT-related proteins caused by AP3S1 knockdown. C Pirfenidone further exacerbates the decreased expression of TGF-β pathway and EMT-related proteins caused by AP3S1 knockdown. All data are mean ± SD. Significance calculated using the unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Representative data are from three independent experiments

Article Snippet: Ovarian cancer cells were subjected to protein extraction for Western blotting analysis after transfection with 80 nM AP3S1-siRNA for 48 h. An activator of the TGF-β signaling pathway known as, SRI-011381 (HY-100347, MCE), was applied at a concentration of 10 μM, and continued treatment was continued for 12 h post-transfection.

Techniques: Inhibition, Knockdown, Expressing